NanoBioPhysik und Medizinische Physik   (Prof. Tilman Schäffer)



N. Schierbaum, J. Rheinlaender, and T.E. Schäffer
Viscoelastic properties of normal and cancerous human breast cells are affected differently by contact to adjacent cells.
Acta Biomaterialia (in press).
Abstract / Link

M. Chatterjee, D. Rath, J. Schlotterbeck, J. Rheinlaender, B. Walker-Allgaier, N. Alnaggar, M. Zdanyte, I. Müller, O. Borst, T. Geisler, T.E. Schäffer, M. Lämmerhofer, and M. Gawaz
Regulation of oxidized platelet lipidome: implications for coronary artery disease.
European Heart Journal (in press).

A. Klapproth, M. Schärli, T. Göen, F. Eckert, T.E. Schäffer, R. Cesnjevar, and F. Münch
Migration von Additiven (DEHP & TOTM) aus Ph.i.s.i.o-beschichteten PVC-Materialien in Medizinprodukten.
Kardiotechnik 1, 3-11 (2017).
Abstract / Link


E. Dubrovin, M. Schächtele, and T.E. Schäffer
Nanotemplate-directed DNA segmental thermal motion.
RSC Advances 6, 79584-79592 (2016).
Abstract / Link

M.S. Salker, N. Schierbaum, N. Alowayed, Y. Singh, A.F. Mack, C. Stournaras, T.E. Schäffer, F. Lang
LeftyA decreases Actin Polymerization and Stiffness in Human Endometrial Cancer Cells.
Scientific Reports 6, 29370 (2016).
Abstract / Link | PDF

M.-J. Kraus, J. Seifert, E.F. Strasser, M. Gawaz, T.E. Schäffer, and J. Rheinlaender
Comparative morphology analysis of live blood platelets using scanning ion conductance and robotic darkfield microscopy.
Platelets 27, 541-546 (2016).
Abstract / Link

O. Schmal, J. Seifert, T.E. Schäffer, C.B. Walter, W.K. Aicher, G. Klein.
Hematopoietic stem and progenitor cell expansion in contact with mesenchymal stromal cells in a hanging drop model uncovers disadvantages of 3D culture.
Stem Cells International, Article ID 4148093, doi:10.1155/2016/4148093 (2016).
Abstract / Link | PDF


J. Rheinlaender and T.E. Schäffer.
Lateral resolution and image formation in scanning ion conductance microscopy.
Analytical Chemistry 87, 7117-7124 (2015).
Abstract / Link

J. Seifert, J. Rheinlaender, P. Novak, Y.E. Korchev, and T.E. Schäffer.
Comparison of atomic force microscopy and scanning ion conductance microscopy for live cell imaging.
Langmuir 31, 6807-6813 (2015).
Abstract / Link

F.M. Hecht, J. Rheinlaender, N. Schierbaum, W.H. Goldmann, B. Fabry, and T.E. Schäffer.
Imaging viscoelastic properties of live cells by AFM: Power-law rheology on the nanoscale.
Soft Matter 11, 4584-4591 (2015).
Abstract / Link | PDF | Cover

S. Vogel, R. Bodenstein, Q. Chen, S. Feil, R. Feil, J. Rheinlaender, T.E. Schäffer, E. Bohn, J.-S. Frick, O. Borst, P. Münzer, B. Walker, J. Markel, G. Csanyi, P.J. Pagano, P. Loughran, M.E. Jessup, S.C. Watkins, G.C. Bullock, J.L. Sperry, B.S. Zuckerbraun, T.R. Billiar, M.T. Lotze, M. Gawaz, and M.D. Neal.
Platelet-derived HMGB1 is a critical mediator of thrombosis.
Journal of Clinical Investigation 125, 4638-4654 (2015).
Abstract / Link

J. Rheinlaender, S. Vogel, J. Seifert, M. Schächtele, O. Borst, F. Lang, M. Gawaz, and T.E. Schäffer.
Imaging the elastic modulus of human platelets during thrombin-induced activation using scanning ion conductance microscopy.
Thrombosis and Haemostasis 113, 305-311 (2015).
Abstract / Link

C.M. Hammer and T.E. Schäffer.
Atomic force microscopy crosslinks interdisciplinary eye research.
Medical Hypothesis, Discovery & Innovation Ophthalmology Journal 4, 1-4 (2015).

K. Metzger, S. Vogel, M. Chatterjee, O. Borst, P. Seizer, T. Schönberger, T. Geisler, F. Lang, H. Langer, J. Rheinlaender, T.E. Schäffer, and M. Gawaz.
High-frequency ultrasound-guided disruption of glycoprotein VI-targeted microbubbles targets atheroprogression in mice.
Biomaterials 36, 80-89 (2015).
Abstract / Link

D. Claus, P.M. Schumacher, M. Wilke, M. Mlikota, U. Weber, S. Schmauder, N. Schierbaum, T.E. Schäffer, P. Wittmüß, T. Teutsch, C. Tarin, S. Hoffmann, S. Brucker, J. Mischinger, C. Schwentner, A. Stenzl, and W. Osten.
Intraoperative model based identification of tissue properties based on multimodal and multiscale measurements.
Proceedings of SPIE 9328, 932805 (2015).
Abstract / Link


C. Braunsmann, J. Seifert, J. Rheinlaender, and T.E. Schäffer.
High-speed force mapping on living cells with a small cantilever atomic force microscope.
Review of Scientific Instruments 85, 073703 (2014).
Abstract / Link | PDF

D. Thatenhorst, J. Rheinlaender, T.E. Schäffer, I.D. Dietzel, and P. Happel.
Effect of sample slope on image formation in scanning ion conductance microscopy.
Analytical Chemistry 86, 9838–9845 (2014).
Abstract / Link

C. Braunsmann, V. Prucker, and T.E. Schäffer.
Optical knife-edge displacement sensor for high-speed atomic force microscopy.
Applied Physics Letters 104, 103101 (2014).
Abstract / Link | PDF

S. Krajewski, J. Rheinlaender, P. Ries, D. Canjuga, C. Mack, L. Scheideler, T.E. Schäffer, J. Geis-Gerstorfer, H.-P. Wendel, and F. Rupp.
Bacterial interactions with proteins and cells relevant for the development of life-threatening endocarditis studied by a quartz crystal microbalance-based biosensor.
Analytical and Bioanalytical Chemistry 406, 3395-3406 (2014).
Abstract / Link

C. Braunsmann and T.E. Schäffer.
Note: Artificial neural networks for the automated analysis of force map data in atomic force microscopy.
Review of Scientific Instruments 85, 056104 (2014).
Abstract / Link | PDF

J. Seifert, C.M. Hammer, J. Rheinlaender, S. Sel, M. Scholz, F. Paulsen, and T.E. Schäffer.
Distribution of the Young’s modulus in porcine corneas after riboflavin/UVA-induced collagen cross-linking by atomic force microscopy.
PLoS ONE 9, e88186 (2014).
Abstract / Link | PDF

C. Braunsmann, R. Proksch, I. Revenko, and T.E. Schäffer.
Creep compliance mapping by atomic force microscopy.
Polymer 55, 219-225 (2014).
Abstract / Link | PDF

S. Hinderer, J. Seifert, M. Votteler, N. Shen, J. Rheinlaender, T.E. Schäffer, and K. Schenke-Layland.
Engineering of a bio-functionalized hybrid off-the-shelf heart valve.
Biomaterials 35, 2130-2139 (2014).
Abstract / Link


T.E. Schäffer.
Nanomechanics of molecules and living cells with scanning ion conductance microscopy.
Analytical Chemistry 85, 6988–6994 (2013).
Abstract / Link | Cover | Audio Interview

J. Rheinlaender and T.E. Schäffer.
Mapping the mechanical stiffness of live cells with the scanning ion conductance microscope.
Soft Matter 9, 3230-3236 (2013).
Abstract / Link | PDF | Cover

M.H. Griessl, B. Schmid, K. Kassler, C. Braunsmann, R. Ritter, B. Barlag, Y.-D. Stierhof, K.U. Sturm, C. Danzer, C. Wagner, T.E. Schäffer, H. Sticht, M. Hensel, and Y.A. Muller.
Structural insight into the giant Ca2+-binding adhesin SiiE: Implications for the adhesion of Salmonella enterica to polarized epithelial cells.
Structure 21, 741-752 (2013).
Abstract / Link

I. Alesutan, J. Seifert, T. Pakladok, J. Rheinlaender, A. Lebedeva, S.T. Towhid, C. Stournaras, J. Voelkl, T.E. Schäffer, and F. Lang.
Chorein sensitivity of actin polymerization, cell shape and mechanical stiffness of vascular endothelial cells.
Cellular Physiology and Biochemistry 32, 728–742 (2013).
Abstract / Link | PDF

W. Xiang, J.C.M. Schlachetzki, S. Heilig, J.C. Bussmann, M. Berlinghof, T.E. Schäffer, K. Marcus, J. Winkler, J. Klucken, and C.-M. Becker.
Oxidative stress-induced posttranslational modifications of alpha-synuclein: Specific modification of alpha-synuclein by 4-hydroxy-2-nonenal increases dopaminergic toxicity.
Molecular and Cellular Neuroscience 54, 71-83 (2013).
Abstract / Link


J. Rheinlaender, A. Gräbner, L. Ott, A. Burkovski, and T.E. Schäffer.
Contour and persistence length of Corynebacterium diphtheriae pili by atomic force microscopy.
European Biophysics Journal 41, 561-570 (2012).
Abstract / Link

C. Braunsmann, C.M. Hammer, J. Rheinlaender, F.E. Kruse, T.E. Schäffer, and U. Schlötzer-Schrehardt.
Evaluation of lamina cribrosa and peripapillary sclera stiffness in pseudo-exfoliation and normal eyes by atomic force microscopy.
Investigative Ophthalmology & Visual Science 53, 2960-2967 (2012)
Abstract / Link


B. Fabry, A.H. Klemm, S. Kienle, T.E. Schäffer, and W.H. Goldmann.
Focal adhesion kinase stabilizes the cytoskeleton.
Biophysical Journal 101, 2131–2138 (2011).
Abstract / Link

J. Rheinlaender, N.A. Geisse, R. Proksch, and T.E. Schäffer.
Comparison of scanning ion conductance microscopy with atomic force microscopy for cell imaging.
Langmuir 27, 697-704 (2011).
Abstract / Link


C. Braunsmann and T.E. Schäffer.
High-speed atomic force microscopy for large scan sizes using small cantilevers.
Nanotechnology 21, 225705 (2010).
Abstract / Link

L. Ott, M. Höller, J. Rheinlaender, T.E. Schäffer, M. Hensel, and A. Burkovski.
Strain-specific differences in pili formation and the interaction of Corynebacterium diphtheriae with host cells.
BMC Microbiology 10:257 (2010).
Abstract / Link | PDF

A.H. Klemm, S. Kienle, J. Rheinlaender, T.E. Schäffer, and W.H. Goldmann.
The Influence of Pyk2 on the Mechanical Properties in Fibroblasts.
Biochemical and Biophysical Research Communications 393, 694-697 (2010).
Abstract / Link

J. Rheinlaender and T.E. Schäffer.
Scanning Ion Conductance Microscopy.
In “Scanning Probe Microscopy of Functional Materials”, S.V. Kalinin and A. Gruverman, eds., Springer Verlag, Berlin, Heidelberg, New York, ISBN 978-1441965677, p.433-460 (2010).

L. Ott, M. Höller, R.G. Gerlach, M. Hensel, J. Rheinlaender, T.E. Schäffer, and A. Burkovski.
Corynebacterium diphtheriae invasion-associated protein (DIP1281) is involved in cell surface organization, adhesion and internalization in epithelial cells.
BMC Microbiology 10:2 (2010).
Abstract / Link | PDF


J. Rheinlaender and T.E. Schäffer.
Image formation and resolution in scanning ion conductance microscopy.
Journal of Applied Physics 105, 094905 (2009).
Abstract / Link | PDF

M. Böcker, S. Muschter, E. Schmitt, C. Steinem, and T.E. Schäffer.
Imaging and patterning of pore-suspending membranes with scanning ion conductance microscopy.
Langmuir 25, 3022-3028 (2009).
Abstract / Link

M. Böcker, H. Fuchs, and T.E. Schäffer.
Scanning Ion Conductance Microscopy of Cellular and Artificial Membranes.
In “Nanotechnology: Volume 6: Nanoprobes”, H. Fuchs, ed., WILEY-VCH Verlag, Weinheim, ISBN 978-3-527-31733-2, p.197-212 (2009).


D. Sánchez, N. Johnson, C. Li, P. Novak, J. Rheinlaender, Y. Zhang, U. Anand, P. Anand, J. Gorelik, G.I. Frolenkov, C. Benham, M. Lab, V.P. Ostanin, T.E. Schäffer, D. Klenerman, and Y.E. Korchev.
Non-contact measurement of the local mechanical properties of living cells using pressure applied via a pipette.
Biophysical Journal 95, 3017–3027 (2008).
Abstract / Link


A. Heredia, C.C. Bui, U. Suter, P. Young, and T.E. Schäffer.
AFM combines functional and morphological analysis of peripheral myelinated and demyelinated nerve fibers.
NeuroImage 37, 1218-1226 (2007).
Abstract / Link

C. Riethmüller, T.E. Schäffer, F. Kienberger, W. Stracke, and H. Oberleithner.
Vacuolar structures can be identified by AFM elasticity mapping.
Ultramicroscopy 107, 895–901 (2007).
Abstract / Link

M. Böcker, B. Anczykowski, J. Wegener, and T.E. Schäffer.
Scanning ion conductance microscopy with distance-modulated shear force control.
Nanotechnology 18, 145505 (2007).
Abstract / Link | Feature on

A. Heredia, M. Aguilar-Franco, C. Magaña, C. Flores, C. Piña, R. Velázquez, T.E. Schäffer, L. Bucio, and V.A. Basiuk.
Structure and interactions of calcite spherulites with alpha-chitin in the brown shrimp (Penaeus aztecus) shell.
Materials Science and Engineering C 27, 8-13 (2007).
Abstract / Link

M. Böcker and T.E. Schäffer.
Scanning Ion Conductance Microscopy.
G.I.T. Imaging & Microscopy 02/2007, 30-32 (2007).

T.E. Schäffer.
Low-noise methods for optical measurements of cantilever deflections.
In “Applied Scanning Probe Methods V”, B. Bhushan, H. Fuchs and S. Kawata, eds., Springer Verlag, Berlin, Heidelberg, New York, ISBN 3-540-37315-2, p.51-74 (2007).

T.E. Schäffer, B. Anczykowski, M. Böcker, and H. Fuchs.
Shear-Force-Controlled Scanning Ion Conductance Microscopy.
In “Nanobiotechnology II: More Concepts and Applications”, C.M. Niemeyer and C.A. Mirkin, eds., WILEY-VCH Verlag, Weinheim, ISBN 978-3-527-31673-1, p.197-212 (2007).


S.M. Cook, T.E. Schäffer, K.M. Chynoweth, M. Wigton, R.W Simmonds, and K.M. Lang.
Practical implementation of dynamic methods for measuring atomic force microscope cantilever spring constants.
Nanotechnology 17, 2135-2145 (2006).
Abstract / Link

T.E. Schäffer.
High-speed atomic force microscopy of biomolecules in motion.
In “Force Microscopy: Applications in Biology and Medicine”, B. Jena, H. Hörber, eds., WILEY-VCH Verlag, Weinheim, ISBN 0-471-39628-1, p. 221-247 (2006).

T.E. Schäffer, B. Anczykowski, and H. Fuchs.
Scanning Ion Conductance Microscopy.
In “Applied Scanning Probe Methods II”, B. Bhushan and H. Fuchs, eds., Springer Verlag, Berlin, Heidelberg, New York, ISBN 3-540-26242-3, p. 91-119 (2006).


T.E. Schäffer.
Calculation of thermal noise in an atomic force microscope with a finite optical spot size.
Nanotechnology 16, 664-670 (2005).
Abstract / Link

S. Schrot, C. Weidenfeller, T.E. Schäffer, H. Robenek, and H.-J. Galla.
Influence of hydrocortisone on the mechanical properties of the cerebral endothelium in vitro.
Biophysical Journal 89, 3904-3910 (2005).
Abstract / Link

T.E. Schäffer and H. Fuchs.
Optimized detection of normal vibration modes of atomic force microscope cantilevers with the optical beam deflection method.
Journal of Applied Physics 97, 083524 1-8 (2005).
Abstract / Link | PDF

V.M. Laurent, S. Kasas, A. Yersin, T.E. Schäffer, S. Catsicas, G. Dietler, A.B. Verkhovsky, and J.-J. Meister.
Gradient of rigidity in the lamellipodia of migrating cells revealed by atomic force microscopy.
Biophysical Journal 89, 667–675 (2005).
Abstract / Link

M. Arnoldi, T.E. Schäffer, M. Fritz, and M. Radmacher.
Direct observation of single catalytic events of chitosanase by atomic force microscopy.
AZojono - Journal of Nanotechnology Online, DOI: 10.2240/azojono0107 (2005).
Abstract / Link

C. Morsczeck, C. Moehl, W. Götz, A. Heredia, T.E. Schäffer, N. Eckstein, C. Sippel, and K.H. Hoffmann.
In vitro differentiation of human dental follicle cells with dexamethasone and insulin.
Cell Biology International 29, 567-575 (2005).
Abstract / Link


Y. Jiao and T.E. Schäffer.
Accurate height and volume measurements on soft samples with the atomic force microscope.
Langmuir 20, 10038-10045 (2004).
Abstract / Link | PDF

R. Proksch, T.E. Schäffer, J.P. Cleveland, R.C. Callahan, and M.B. Viani.
Finite optical spot size and position corrections in thermal spring constant calibration.
Nanotechnology 15, 1344-1350 (2004).
Abstract / Link


T.E. Schäffer, M. Radmacher, and R. Proksch.
Magnetic force gradient mapping.
Journal of Applied Physics 94, 6525-6532 (2003).
Abstract / Link | PDF

V. Ivanova, I. Makarov, T.E. Schäffer, and T. Heimburg.
Analyzing heat capacity profiles of peptide-containing membranes: Cluster formation of Gramicidin A.
Biophysical Journal 84, 2427-2439 (2003).
Abstract / Link


T.E. Schäffer.
Force Spectroscopy with a Large Dynamic Range Using Small Cantilevers and an Array Detector.
Journal of Applied Physics 91, 4739-4746 (2002).
Abstract / Link | PDF

A. Hoff, T. André, T.E. Schäffer, G. Jung, K.-H. Wiesmüller, and R. Brock.
Lipoconjugates for the Noncovalent Generation of Microarrays in Biochemical and Cellular Assays.
ChemBioChem 3, 1183-1191 (2002).
Abstract / Link


Y. Jiao, D.I. Cherny, G. Heim, T.M. Jovin, and T.E. Schäffer.
Dynamic Interactions of p53 with DNA in Solution by Time-Lapse Atomic Force Microscopy.
Journal of Molecular Biology 314, 233-243 (2001).
Abstract / Link

G. Hemakanthi, A. Dhathathreyan, B.U. Nair, T. Ramasami, D. Möbius, and T.E. Schäffer.
Control of size of PbI2 nanocrystals using Langmuir-Blodgett films of n-octadecyl succinic acid.
Colloids and Surfaces A, 181, 115-121 (2001).
Abstract / Link


T.E. Schäffer, M. Richter, and M.B. Viani.
Array Detector for the Atomic Force Microscope.
Applied Physics Letters 76, 3644-3646 (2000).
Abstract / Link | PDF

S. Kasas, N.H. Thomson, T.E. Schäffer, G. Dietler, S. Catsicas, and P.K. Hansma.
Simulation of an atomic force microscope imaging a moving protein.
Probe Microscopy 2, 37-44 (2000).

A. Chand, M.B. Viani, T.E. Schäffer, and P.K. Hansma.
Microfabricated Small Metal Cantilevers with Silicon Tips for Atomic Force Microscopy.
Journal of Microelectromechanical Systems 9, 112-116 (2000).
Abstract / Link


B.L. Smith*, T.E. Schäffer*, M. Viani, J.B. Thompson, N.A. Frederick, J. Kindt, A. Belcher, G.D. Stucky, D.E. Morse, and P.K. Hansma. (* equal contribution)
Molecular Mechanistic Origin of the Toughness of Natural Adhesives, Fibres and Composites.
Nature 399, 761-763 (1999).
Abstract / Link

M.B. Viani, T.E. Schäffer, A. Chand, M. Rief, H.E. Gaub, and P.K. Hansma.
Small Cantilevers for Force Spectroscopy of Single Molecules.
Journal of Applied Physics 86, 2258-2262 (1999).
Abstract / Link

M.B. Viani, T.E. Schäffer, G.T. Paloczi, L. Pietrasanta, B.L. Smith, J.B. Thompson, M. Richter, M. Rief, H.E. Gaub, K.W. Plaxco, A.N. Cleland, H.G. Hansma, and P.K. Hansma.
Fast imaging and fast force spectroscopy of single biopolymers with a new atomic force microscope designed for small cantilevers.
Review of Scientific Instruments 70, 4300-4303 (1999).
Abstract / Link

T.E. Schäffer.
Neue Kraftmikroskope mit Anwendung in der Biologie.
MPIbpc News 05/1999, 1-6 (1999).


T.E. Schäffer and P.K. Hansma.
Characterization and Optimization of the Detection Sensitivity of an Atomic Force Microscope for Small Cantilevers.
Journal of Applied Physics 84, 4661-4666 (1998).
Abstract / Link | PDF


T.E. Schäffer, C. Ionescu-Zanetti, R. Proksch, M. Fritz, D.A. Walters, N. Almqvist, C.M. Zaremba, A.M. Belcher, B.L. Smith, G.D. Stucky, D.E. Morse, and P.K. Hansma.
Does Abalone Nacre Form by Heteroepitaxial Nucleation or by Growth through Mineral Bridges?" Chemistry of Materials 9, 1731-1740 (1997). (Was discussed in "news and views", Nature 389, 912-915 (1997).)
Abstract / Link

T.E. Schäffer, M. Viani, D.A. Walters, B. Drake, E.K. Runge, J.P. Cleveland, M.A. Wendman, and P.K. Hansma.
An Atomic Force Microscope for Small Cantilevers.
Proceedings of the SPIE 3009, 48-52 (1997).

S. Manne, T.E. Schäffer, Q. Huo, P.K. Hansma, D.E. Morse, G.D. Stucky, and I.A. Aksay.
Gemini Surfactants at Solid-Liquid Interfaces: Control of Interfacial Aggregate Geometry.
Langmuir 13, 6382-6387 (1997).
Abstract / Link

S.H. Tolbert, T.E. Schäffer, J. Feng, P.K. Hansma, and G.D. Stucky.
A New Phase of Oriented Mesoporous Silicate Thin Films.
Chemistry of Materials 9, 1962-1967 (1997).
Abstract / Link

D.A. Walters, M. Viani, G.T. Paloczi, T.E. Schäffer, J.P. Cleveland, M.A. Wendman, G. Gurley, V. Elings, and P.K. Hansma.
Atomic Force Microscopy using Small Cantilevers.
Proceedings of the SPIE 3009, 43-47 (1997).


T.E. Schäffer, J.P. Cleveland, F. Ohnesorge, D.A. Walters, and P.K. Hansma.
Studies of Vibrating Atomic Force Microscope Cantilevers in Liquid.
Journal of Applied Physics 80, 3622-3627 (1996).
Abstract / Link | PDF


J.P. Cleveland, T.E. Schäffer, and P.K. Hansma.
Probing Oscillatory Hydration Potentials using Thermal-Mechanical Noise in an Atomic Force Microscope.
Physical Review B: Rapid Communications 52, R8692-R8695 (1995).
Abstract / Link

J.P. Cleveland, T.E. Schäffer, and P.K. Hansma.
Measuring Intermolecular Forces using Thermal Noise in an Atomic Force Microscope.
Proc. Microscopy and Microanalysis, Jones and Begell Publishing, New York, 724-725 (1995).

R. Proksch, T.E. Schäffer, B.M. Moskowitz, E.D. Dahlberg, D.A. Bazylinski, and R.B. Frankel.
Magnetic Force Microscopy of the Submicron Magnetic Assembly in a Magnetotactic Bacterium.
Applied Physics Letters 66, 2582-2584 (1995).
Abstract / Link

J. Shi, J.M. Kikkawa, R. Proksch, T.E. Schäffer, D.D. Awschalom, G. Medeiros-Ribeiro, and P.M. Petroff.
Assembly of Submicrometre Ferromagnets in Gallium Arsenide Semiconductors.
Nature 377, 707-710 (1995).
Abstract / Link

Copyright information for AIP (American Institute of Physics) articles: Copyright American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics.